Localization of Chitin Synthase in Mucov vouxii by an Autoradiographic
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چکیده
The localization of chitin synthase in the cells of Mucor rouxii was studied by a method which combined permeabilization of the cells with toluene/ethanol and incubation with the radioactive substrate UDP-[3H]GlcNAc followed by high resolution autoradiography. By this technique it was demonstrated that most of the chitin synthesized by these cells was located within the cytoplasm, and only a small amount of the enzyme product appeared at the cell surface. It was concluded that most of the chitin synthase of M. rouxii is located in the cytoplasm of the cells.
منابع مشابه
Pathway of chitin synthesis and cellular localization of chitin synthetase in Mocor rouxii.
Cell-free extracts of ikfucor rouxii contain enzymes which synthesize uridine diphosphate &lcetyl-D-glucosamine from IV-acetyl-n-glucosamine. Procedures are described to prepare radiolabeled UDP-GlcNAc using cell-free extracts or living cells of this fungus. This sugar nucleotide serves as the precursor for chitin synthesis. Free N-acetyl-n-glucosamine is a strong activator of chitin synthesis....
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تاریخ انتشار 1983